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PTM-POSE

Overview:

  • Overview
  • Dependencies
  • PTM-POSE Reference

Instructions:

  • Installation
  • Running PTM-POSE
  • Filtering PTMs and Events
  • Annotating PTMs with Functional Information

Example Vignettes

  • Examples
    • ESRP1 Knockdown (MATS output)
    • ESRP1 Expression in Prostate Cancer (TCGASpliceSeq output)

Analysis Gallery

  • Gallery
    • General Overview of Spliced PTMs
      • Inspecting types of PTMs impacted by splicing
      • Inspecting number of PTMs with annotation information available
      • Impact of filtering PTMs
    • Functional Impact of Spliced PTMs
      • Identifying available PTM-specific annotation information
      • Identify enriched gene sets associated with differentially spliced PTMs
      • Assessing enriched PTM functions
    • Impact on protein interaction and regulatory networks
      • Kinase substrate enrichment analysis (KSEA)
      • Protein interactions network
      • Identify kinases with enriched substrates in differentially included exons, using an adapted version of KSTAR
    • Analyzing altered flanking sequences
      • Inspect difference of flanking sequence due to splice event
      • Identify altered SH2 domain motifs
      • Probing where and how PTM flanking sequences are altered
      • Kinase affinity due to PTM flanking sequence alterations

Troubleshooting

  • Frequently Asked Questions
  • .rst

Gallery

Contents

  • General Overview of Spliced PTMs
  • Functional Impact of Spliced PTMs
  • Impact on protein interaction and regulatory networks
  • Analyzing altered flanking sequences

Gallery#

Below you will find different ways you might choose to analyze the PTMs identified by PTM-POSE:

General Overview of Spliced PTMs#

Inspecting types of PTMs impacted by splicing

Inspecting types of PTMs impacted by splicing

Inspecting number of PTMs with annotation information available

Inspecting number of PTMs with annotation information available

Impact of filtering PTMs

Impact of filtering PTMs

Functional Impact of Spliced PTMs#

Identifying available PTM-specific annotation information

Identifying available PTM-specific annotation information

Identify enriched gene sets associated with differentially spliced PTMs

Identify enriched gene sets associated with differentially spliced PTMs

Assessing enriched PTM functions

Assessing enriched PTM functions

Impact on protein interaction and regulatory networks#

Kinase substrate enrichment analysis (KSEA)

Kinase substrate enrichment analysis (KSEA)

Protein interactions network

Protein interactions network

Identify kinases with enriched substrates in differentially included exons, using an adapted version of KSTAR

Identify kinases with enriched substrates in differentially included exons, using an adapted version of KSTAR

Analyzing altered flanking sequences#

Inspect difference of flanking sequence due to splice event

Inspect difference of flanking sequence due to splice event

Identify altered SH2 domain motifs

Identify altered SH2 domain motifs

Probing where and how PTM flanking sequences are altered

Probing where and how PTM flanking sequences are altered

Kinase affinity due to PTM flanking sequence alterations

Kinase affinity due to PTM flanking sequence alterations

Download all examples in Python source code: gallery_output_python.zip

Download all examples in Jupyter notebooks: gallery_output_jupyter.zip

Gallery generated by Sphinx-Gallery

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ESRP1 Expression in Prostate Cancer (TCGASpliceSeq output)

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General Overview of Spliced PTMs

Contents
  • General Overview of Spliced PTMs
  • Functional Impact of Spliced PTMs
  • Impact on protein interaction and regulatory networks
  • Analyzing altered flanking sequences

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